タカハシ　リョウヤ   Takahashi Ryoya   高橋　良哉    所属   東邦大学  薬学部 薬学科    職種   教授
言語種別	日本語
発表タイトル	Altered expression of myelin basic protein gene in the cerebellum of SAMP8 mice.
会議名	第31回日本基礎老化学会
学会区分	国内学会
発表形式	ポスター掲示
講演区分	一般
発表者・共同発表者	◎Takahashi R†
発表年月日	2008/06/13
開催地 （都市, 国名）	松本
概要	Myelin basic protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. MBP transcripts can be detected as early as 2 days after birth in the mouse, peak at day 18, and progressively decrease to about 20% of the peak level in the mature animal. In both SAMP8 and SAMR1, MBP mRNA was first detected in all regions of the brain examined at day 4, increased dramatically to a peak at about day16 and then gradually decreased in both strains with advancing age (day 30 to 50). In the cerebrum and brain stem, no appreciable change in the MBP mRNA level was observed between the two strains at any age. In the cerebellum, however, the increased MBP mRNA level with age in SAMP8 began to decline at 20 days when the mRNA level was still relatively high in SAMR1. To determine whether the decline of MBP mRNA with age in the SAMP8 strain is due to certain MBP mRNA splicing variant, we conducted RT-PCR using specific primers. Five different mRNA variants were observed in the cerebellum of both SAMP8 and SAMR1, suggesting that the decline of MBP mRNAs due to the decease of transcriptional activity of MBP gene rather than the alteration of splicing process. Regardless of the mechanism of alteration of MBP gene expression, the decline of MBP mRNA level in the cerebellum of SAMP8 after weaning might cause impaired myelin formation that leads to the learning-memory deficit which is observed early in life. [Keywords] myelin basic protein, alternative splicing, senescence accelerated mouse, cerebellum, SAMP8