スギモト　マサズミ   Sugimoto Masazumi   杉本　雅純    所属   東邦大学  理学部 生物分子科学科    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Norepinephrine induces apoptosis in skin melanophores by attenuating cAMP-PKA signals via alpha2-adrenoceptors in the medaka, Oryzias latipes
掲載誌名	正式名：Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society 略　 称：Pigment Cell Res ISSNコード：08935785/16000749
巻・号・頁	14(5),pp.356-361
著者・共著者	Uchida-Oka N, Sugimoto M
担当区分	最終著者,責任著者
発行年月	2001/10
概要	The density of skin melanophores in many teleost fish decreases during long-term adaptation to a white background. Using the medaka, Oryzias latipes, we previously reported that apoptosis is responsible for the decrease in melanophores, and that a sympathetic neurotransmitter, norepinephrine (NE), induces their apoptosis in skin tissue cultures. In this study, we show that NE-induced apoptosis of melanophores is mediated by the activation of alpha2-adrenoceptors. Clonidine, an alpha2-adrenoceptor agonist, induced apoptotic melanophore death in skin organ culture, while phenylephrine, an alpha1-adrenoceptor agonist, had no effect. NE-induced apoptosis was diminished by an alpha2-adrenoceptor antagonist, yohimbine, but an alpha1-adrenoceptor antagonist, prazosin, did not abrogate the effect of NE. Furthermore, forskolin inhibited NE-induced apoptosis, while an inhibitor of PKA, H- 89, mimicked the effect of NE. These results suggest that NE induces apoptosis in melanophores by attenuating cAMP-PKA signaling via alpha2- adrenoceptors.