スギモト マサズミ   Sugimoto Masazumi
  杉本 雅純
   所属   東邦大学  理学部 生物分子科学科
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Norepinephrine induces apoptosis in skin melanophores by attenuating cAMP-PKA signals via alpha2-adrenoceptors in the medaka, Oryzias latipes
掲載誌名 正式名:Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society
略  称:Pigment Cell Res
ISSNコード:08935785/16000749
巻・号・頁 14(5),356-361頁
著者・共著者 Uchida-Oka N, Sugimoto M
発行年月 2001/10
概要 The density of skin melanophores in many teleost fish decreases during long-term adaptation to a white background. Using the medaka, Oryzias latipes, we previously reported that apoptosis is responsible for the decrease in melanophores, and that a sympathetic neurotransmitter, norepinephrine (NE), induces their apoptosis in skin tissue cultures. In this study, we show that NE-induced apoptosis of melanophores is mediated by the activation of alpha2-adrenoceptors. Clonidine, an alpha2-adrenoceptor agonist, induced apoptotic melanophore death in skin organ culture, while phenylephrine, an alpha1-adrenoceptor agonist, had no effect. NE-induced apoptosis was diminished by an alpha2-adrenoceptor antagonist, yohimbine, but an alpha1-adrenoceptor antagonist, prazosin, did not abrogate the effect of NE. Furthermore, forskolin inhibited NE-induced apoptosis, while an inhibitor of PKA, H- 89, mimicked the effect of NE. These results suggest that NE induces apoptosis in melanophores by attenuating cAMP-PKA signaling via alpha2- adrenoceptors.