オバラ　チズカ   Obara Chizuka   小原　千寿香    所属   東邦大学  理学部 生物学科    職種   博士研究員
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Development of a three-dimensional bioprinter: construction of cell supporting structures using hydrogel and state-of-the-art inkjet technology.
掲載誌名	正式名：Journal of biomechanical engineering ISSNコード：0148-0731
掲載区分	国外
出版社	ASME-AMER SOC MECHANICAL ENG
巻・号・頁	131(3),pp.035001-035001
著者・共著者	Yuichi Nishiyama,Makoto Nakamura,Chizuka Henmi,Kumiko Yamaguchi,Shuichi Mochizuki,Hidemoto Nakagawa,Koki Takiura
発行年月	2009/03
概要	We have developed a new technology for producing three-dimensional (3D) biological structures composed of living cells and hydrogel in vitro, via the direct and accurate printing of cells with an inkjet printing system. Various hydrogel structures were constructed with our custom-made inkjet printer, which we termed 3D bioprinter. In the present study, we used an alginate hydrogel that was obtained through the reaction of a sodium alginate solution with a calcium chloride solution. For the construction of the gel structure, sodium alginate solution was ejected from the inkjet nozzle (SEA-Jet (TM), Seiko Epson Corp., Suwa, Japan) and was mixed with a substrate composed of a calcium chloride solution. In our 3D bioprinter, the nozzle head can be moved in three dimensions. Owing to the development of the 3D bioprinter, an innovative fabrication method that enables the gentle and precise fixation of 3D gel structures was established using living cells as a material. To date, several 3D structures that include living cells have been fabricated, including lines, planes, laminated structures, and tubes, and now, experiments to construct various hydrogel structures are being carried out in our laboratory.
DOI	10.1115/1.3002759
PMID	19154078