ミカミ　ヨシノリ   Mikami Yoshinori   三上　義礼    所属   東邦大学  医学部 医学科    職種   助教
言語種別	英語
発表タイトル	Polysulfides derived from H2S, are possible signaling molecules that activate TRPA1 channels in rat brain
会議名	第38回日本神経科学大会
学会区分	国内学会
発表形式	口頭
講演区分	一般
発表者・共同発表者	◎木村由佳, 三上義礼†, 大隅貴美子, 津金麻美子, 岡淳一郎, 木村英雄
発表年月日	2015/07/28
開催地 （都市, 国名）	神戸
概要	Hydrogen sulfide (H2S) is established as a bioactive substance with various physiological functions. H2S, which is enzymatically produced from cysteine,is oxidized to produce polysulfides (H2Sn,n>2). We previously reported that polysulfides induce Ca2+ influx in astrocytes more potently than H2S. However, the receptor that mediates the response has not been identified. Here, we have shown that polysulfides induce Ca2+ influx by activating transient receptor potential ankyrin-1 (TRPA1) channels in rat astrocytes. The response induced by 0.5 μM polysulfide requires approximately 300 times greater concentrations of H2S. TRPA1-selective agonists, allyl isothiocyanate and cinnamaldehyde, induced Ca2+ influx, and responses to polysulfides were suppressed by TRPA1-selective inhibitors, HC-030031 and AP-18, as well as by siRNAs selective to TRPA1. The present study suggests that polysulfides are potential H2S-derived signaling molecules that stimulate TRPA1 channels in the brain. Above study also suggests that some of the functions ascribes to H2S are mediated by polysulfides.