ヤマダ　ヨウコ   Yamada Yoko   山田　葉子    所属   東邦大学  理学部 生物学科    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読なし
表題	Loss of PIKfyve Causes Transdifferentiation of Dictyostelium Spores Into Basal Disc Cells.
掲載誌名	正式名：Frontiers in cell and developmental biology
掲載区分	国外
巻・号・頁	9,pp.692473-692473
著者・共著者	Yoko Yamada,Gillian Forbes,Qingyou Du,Takefumi Kawata,Pauline Schaap
発行年月	2021
概要	The 1-phosphatidylinositol-3-phosphate 5-kinase PIKfyve generates PtdIns3,5P2 on late phagolysosomes, which by recruiting the scission protein Atg18, results in their fragmentation in the normal course of endosome processing. Loss of PIKfyve function causes cellular hypervacuolization in eukaryotes and organ failure in humans. We identified pikfyve as the defective gene in a Dictyostelium mutant that failed to form spores. The amoebas normally differentiated into prespore cells and initiated spore coat protein synthesis in Golgi-derived prespore vesicles. However, instead of exocytosing, the prespore vesicles fused into the single vacuole that typifies the stalk and basal disc cells that support the spores. This process was accompanied by stalk wall biosynthesis, loss of spore gene expression and overexpression of ecmB, a basal disc and stalk-specific gene, but not of the stalk-specific genes DDB_G0278745 and DDB_G0277757. Transdifferentiation of prespore into stalk-like cells was previously observed in mutants that lack early autophagy genes, like atg5, atg7, and atg9. However, while autophagy mutants specifically lacked cAMP induction of prespore gene expression, pikfyve - showed normal early autophagy and prespore induction, but increased in vitro induction of ecmB. Combined, the data suggest that the Dictyostelium endosomal system influences cell fate by acting on cell type specific gene expression.
DOI	10.3389/fcell.2021.692473
PMID	34490246