フルタ トシアキ   Furuta Toshiaki
  古田 寿昭
   所属   東邦大学  理学部 生物分子科学科
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells
掲載誌名 正式名:Chemical Communications
巻・号・頁 50(6),pp.664-666
著者・共著者 Teraoka A, Murakoshi K, Fukamauchi K, Suzuki AZ, Watanabe S†, Furuta T*†
担当区分 最終著者,責任著者
発行年月 2014/01
概要 A facile and useful method for preparing caged DNAs was developed. The method includes a caging reaction of a linear dsDNA having a minimal sequence of protein expression with Bio-Bhc-diazo and affinity separation of the caged DNA. Effective suppression and photo-mediated restoration of protein expression were demonstrated.A facile and useful method for preparing caged DNAs was developed. The method includes a caging reaction of a linear dsDNA having a minimal sequence of protein expression with Bio-Bhc-diazo and affinity separation of the caged DNA. Effective suppression and photo-mediated restoration of protein expression were demonstrated.