ホソイ　ハルコ   Hosoi Haruko   細井　晴子    所属   東邦大学  理学部 生物分子科学科    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Excited-State Proton Transfer Dynamics in LSSmOrange Studied by Time-Resolved Impulsive Stimulated Raman Spectroscopy
掲載誌名	正式名：The Journal of Physical Chemistry Letters 略　 称：J. Phys. Chem. Lett. ISSNコード：19487185
掲載区分	国外
出版社	American Chemical Society
巻・号・頁	12(31),pp.7466-7473
総ページ数	8
著者・共著者	Pardeep Kumar, Eduard Fron, Haruko Hosoi, Hikaru Kuramochi, Satoshi Takeuchi, Hideaki Mizuno, Tahei Tahara
発行年月	2021/08/02
概要	LSSmOrange is a fluorescent protein that exhibits a large energy gap between absorption and emission, which makes it a useful tool for multicolor bioimaging. This characteristic of LSSmOrange originates from excited-state proton transfer (ESPT): The neutral chromophore is predominantly present in the ground state while the bright fluorescence is emitted from the anionic excited state after ESPT. Interestingly, it was reported that this ESPT process follows bimodal dynamics, but its origin has not clearly been understood. We investigate ESPT of LSSmOrange using time-resolved impulsive stimulated Raman spectroscopy (TR-ISRS) that provides femtosecond time-resolved Raman spectra. The results indicate that the bimodal ESPT dynamics originates from the structural heterogeneity of the chromophore. Species-associated Raman spectra obtained by spectral analysis based on singular value decomposition (SVD) suggest that cis and trans chromophores coexist in the ground state. It is considered that these two forms are photoexcited and undergo ESPT in parallel, resulting in the bimodal dynamics of ESPT in LSSmOrange.