ホソイ ハルコ   Hosoi Haruko
  細井 晴子
   所属   東邦大学  理学部 生物分子科学科
   職種   准教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Role of Coherent Low-Frequency Motion in Excited-State Proton Transfer of Green Fluorescent Protein Studied by Time-Resolved Impulsive Stimulated Raman Spectroscopy
掲載誌名 正式名:Journal of the American Chemical Society
略  称:J. Am. Chem. Soc.
ISSNコード:00027863
出版社 American Chemical Society
巻・号・頁 138(12),3942-3945頁
著者・共著者 T. Fujisawa, H. Kuramochi, H. Hosoi, S. Takeuchi, and T. Tahara
発行年月 2016/03
概要 Green fluorescent protein (GFP) from
jellyfish Aequorea victoria, an essential bioimaging tool,
luminesces via excited-state proton transfer (ESPT) in
which the phenolic proton of the p-hydroxybenzylideneimidazolinone
chromophore is transferred to Glu222
through a hydrogen-bond network. In this process, the
ESPT mediated by the low-frequency motion of the
chromophore has been proposed. We address this issue
using femtosecond time-resolved impulsive stimulated
Raman spectroscopy. After coherently exciting lowfrequency
modes (<300 cm−1) in the excited state of
GFP, we examined the excited-state structural evolution
and the ESPT dynamics within the dephasing time of the
low-frequency vibration. A clear anharmonic vibrational
coupling is found between one high-frequency mode of
the chromophore (phenolic CH bend) and a lowfrequency
mode at ∼104 cm−1. However, the data show
that this low-frequency motion does not substantially
affect the ESPT dynamics.
DOI 10.1021/jacs.5b11038