ホソイ　ハルコ   Hosoi Haruko   細井　晴子    所属   東邦大学  理学部 生物分子科学科    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Role of Coherent Low-Frequency Motion in Excited-State Proton Transfer of Green Fluorescent Protein Studied by Time-Resolved Impulsive Stimulated Raman Spectroscopy
掲載誌名	正式名：Journal of the American Chemical Society 略　 称：J. Am. Chem. Soc. ISSNコード：00027863
出版社	American Chemical Society
巻・号・頁	138(12),pp.3942-3945
著者・共著者	T. Fujisawa, H. Kuramochi, H. Hosoi, S. Takeuchi, and T. Tahara
発行年月	2016/03
概要	Green fluorescent protein (GFP) from jellyfish Aequorea victoria, an essential bioimaging tool, luminesces via excited-state proton transfer (ESPT) in which the phenolic proton of the p-hydroxybenzylideneimidazolinone chromophore is transferred to Glu222 through a hydrogen-bond network. In this process, the ESPT mediated by the low-frequency motion of the chromophore has been proposed. We address this issue using femtosecond time-resolved impulsive stimulated Raman spectroscopy. After coherently exciting lowfrequency modes (<300 cm−1) in the excited state of GFP, we examined the excited-state structural evolution and the ESPT dynamics within the dephasing time of the low-frequency vibration. A clear anharmonic vibrational coupling is found between one high-frequency mode of the chromophore (phenolic CH bend) and a lowfrequency mode at ∼104 cm−1. However, the data show that this low-frequency motion does not substantially affect the ESPT dynamics.
DOI	10.1021/jacs.5b11038