カジタニ　タカシ   梶谷　宇    所属   東邦大学  医学部 医学科    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	CD34 and CD49f Double-Positive and Lineage Marker-Negative Cells Isolated from Human Myometrium Exhibit Stem Cell-Like Properties Involved in Pregnancy-Induced Uterine Remodeling.
掲載誌名	正式名：Biology of reproduction
掲載区分	国外
巻・号・頁	93(2),pp.37-37
著者・共著者	Masanori Ono,Takashi Kajitani,Hiroshi Uchida,Toru Arase,Hideyuki Oda,Sayaka Uchida,Kuniaki Ota,Takashi Nagashima,Hirotaka Masuda,Kaoru Miyazaki,Hironori Asada,Naoko Hida,Yo Mabuchi,Satoru Morikawa,Mamoru Ito,Serdar E Bulun,Hideyuki Okano,Yumi Matsuzaki,Yasunori Yoshimura,Tetsuo Maruyama
発行年月	2015/08
概要	Repeated and dramatic pregnancy-induced uterine enlargement and remodeling throughout reproductive life suggests the existence of uterine smooth muscle stem/progenitor cells. The aim of this study was to isolate and characterize stem/progenitor-like cells from human myometrium through identification of specific surface markers. We here identify CD49f and CD34 as markers to permit selection of the stem/progenitor cell-like population from human myometrium and show that human CD45(-) CD31(-) glycophorin A(-) and CD49f(+) CD34(+) myometrial cells exhibit stem cell-like properties. These include side population phenotypes, an undifferentiated status, high colony-forming ability, multilineage differentiation into smooth muscle cells, osteoblasts, adipocytes, and chondrocytes, and in vivo myometrial tissue reconstitution following xenotransplantation. Furthermore, CD45(-) CD31(-) glycophorin A(-) and CD49f(+) CD34(+) myometrial cells proliferate under hypoxic conditions in vitro and, compared with the untreated nonpregnant myometrium, show greater expansion in the estrogen-treated nonpregnant myometrium and further in the pregnant myometrium in mice upon xenotransplantation. These results suggest that the newly identified myometrial stem/progenitor-like cells influenced by hypoxia and sex steroids may participate in pregnancy-induced uterine enlargement and remodeling, providing novel insights into human myometrial physiology.
DOI	10.1095/biolreprod.114.127126
PMID	26108791