セキ　タカオ   Seki Takao   関　崇生    所属   東邦大学  医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
招待の有無	招待あり
表題	Protocol to isolate quiescent hepatic stellate cells in sufficient yield while maintaining cytokine responsiveness from aged female mice.
掲載誌名	正式名：STAR Protocols
掲載区分	国外
出版社	Cell Press
巻・号・頁	6(4)
総ページ数	19
著者・共著者	Takao Seki, Sachiko Komazawa-Sakon, Yuichi Tsuchiya, Kyoko Fukuhara, Yasuhiro Nakano, Kenichi Ikejima, Katsuhide Okunishi, Hiroyasu Nakano
担当区分	筆頭著者,責任著者
発行年月	2025/12/10
概要	Isolation of primary hepatic stellate cells (HSCs) in sufficient quantities is technically challenging. We present a protocol for isolating quiescent HSCs from mice using Nycodenz density-gradient centrifugation. The procedure includes cervical dislocation, vena cava cannulation, liver perfusion, enzymatic digestion, and preparation for density separation. We then describe centrifugation, enrichment, washing, counting, and culture preparation. This protocol provides fresh HSCs suitable for diverse experimental applications. For complete details on the use and execution of this protocol, please refer to Seki et al.
researchmap用URL	https://www.sciencedirect.com/science/article/pii/S2666166725006720?via%3Dihub