ツチヤ　ユウイチ   Tsuchiya Yuuichi   土屋　勇一    所属   東邦大学  薬学部 薬学科    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
招待の有無	招待あり
表題	Protocol to isolate quiescent hepatic stellate cells in sufficient yield while maintaining cytokine responsiveness from aged female mice.
掲載誌名	正式名：STAR Protocols
掲載区分	国外
出版社	Cell Press
巻・号・頁	6(4)
総ページ数	19
著者・共著者	Takao Seki, Sachiko Komazawa-Sakon, Yuichi Tsuchiya, Kyoko Fukuhara, Yasuhiro Nakano, Kenichi Ikejima, Katsuhide Okunishi, Hiroyasu Nakano
発行年月	2025/12/10
概要	Isolation of primary hepatic stellate cells (HSCs) in sufficient quantities is technically challenging. We present a protocol for isolating quiescent HSCs from mice using Nycodenz density-gradient centrifugation. The procedure includes cervical dislocation, vena cava cannulation, liver perfusion, enzymatic digestion, and preparation for density separation. We then describe centrifugation, enrichment, washing, counting, and culture preparation. This protocol provides fresh HSCs suitable for diverse experimental applications. For complete details on the use and execution of this protocol, please refer to Seki et al.
researchmap用URL	https://www.sciencedirect.com/science/article/pii/S2666166725006720?via%3Dihub