|
オクニシ カツヒデ
Okunishi Katsuhide
奥西 勝秀 所属 東邦大学 医学部 医学科 職種 教授 |
|
| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | In vivo IL-10 gene delivery suppresses airway eosinophilia and hyperreactivity by down-regulating APC functions and migration without impairing the antigen-specific systemic immune response in a mouse model of allergic airway inflammation. |
| 掲載誌名 | 正式名：Journal of immunology (Baltimore, Md. : 1950) ISSNコード：0022-1767 |
| 掲載区分 | 国外 |
| 出版社 | AMER ASSOC IMMUNOLOGISTS |
| 巻・号・頁 | 174(11),pp.6955-66 |
| 著者・共著者 | Kazuyuki Nakagome,Makoto Dohi,Katsuhide Okunishi,Yoshinori Komagata,Katsuya Nagatani,Ryoichi Tanaka,Jun-ichi Miyazaki,Kazuhiko Yamamoto |
| 発行年月 | 2005/06/01 |
| 概要 | IL-10 is an immunosuppressive cytokine. Although previous studies have reported that exogenous delivery of IL-10 reduced airway inflammation in experimental allergic airway inflammation, the mechanism of action has not been fully clarified. In this report, we elucidated a mechanism of action of IL-10 in vivo. BALB/c mice were immunized and aerosol challenged with OVA-Ag. We delivered the IL-10 gene to the mice before systemic sensitization or during aerosol Ag challenge by administering an IL-10-producing plasmid vector. Not only presensitization delivery of IL-10, as reported, but also delivery during inflammation strongly suppressed the development of airway eosinophilia and hyperreactivity. Presensitization delivery suppressed the Ag-specific Th2-type immune response in both the lung and spleen. In contrast, delivery in the effector phase suppressed the Th2 response only in the lung, whereas that in the spleen was not affected. IL-10 gene delivery did not induce the development of a regulatory phenotype of T cells or dendritic cells; rather, it suppressed the overall functions of CD11c(+) APCs of the lung such as Ag-presenting capacity, cytokine production, and transportation of OVA-Ag to lymph nodes, thus attenuating Th2-mediated allergic airway inflammation. Further, IL-10 revealed a distinct immunosuppressive effect in the presence of Ag and APCs. These results suggest that suppression of APC function in the lung, the site of immune response, played a critical role in the IL-10-mediated suppression of Ag-induced airway inflammation and hyperreactivity. Therefore, if delivered selectively, IL-10 could site specifically suppress the Ag-specific immune response without affecting systemic immune responses. |
| DOI | 10.4049/jimmunol.174.11.6955 |
| PMID | 15905538 |