オクニシ　カツヒデ   Okunishi Katsuhide   奥西　勝秀    所属   東邦大学  医学部 医学科    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Noninvasive system for evaluating allergen-induced nasal hypersensitivity in murine allergic rhinitis.
掲載誌名	正式名：Laboratory investigation; a journal of technical methods and pathology ISSNコード：0023-6837
掲載区分	国外
出版社	NATURE PUBLISHING GROUP
巻・号・頁	86(9),pp.917-26
著者・共著者	Muneo Nakaya,Makoto Dohi,Katsuhide Okunishi,Kazuyuki Nakagome,Ryoichi Tanaka,Mitsuru Imamura,Shintaro Baba,Naonobu Takeuchi,Kazuhiko Yamamoto,Kimitaka Kaga
発行年月	2006/09
概要	Until now there has been no method for physiologically evaluating nasal hypersensitivity in mice. Enhanced pause (Penh) has been used as an indicator that reflects changes in the lower airway. Recently, however, there is disagreement regarding the significance of the Penh system; this is because Penh is not essentially a physiological parameter, and it might not necessarily represent a change in the lower respiratory tract. The purpose of the present study is to investigate whether Penh could be applicable for analyzing nasal hypersensitivity in mice. BALB/c mice were sensitized with ovalbumin (OVA) through a combination of intraperitoneal injection and daily intranasal challenge in an awake condition. Penh was measured at each time point during sensitization, or a serial change in Penh value was followed after the final nasal challenge and the effect of treatment was assessed. Following sensitization and nasal challenge, the Penh value gradually increased and showed a significant difference on day 14. Changes in IgE, eosinophil infiltration into nasal mucosa, and OVA-induced symptoms all strongly correlated with the increase in Penh. On day 19, after OVA nasal provocation, Penh gradually increased and reached maximal values 25 min after the challenge. Pretreatment with dexamethasone or a histamine H1 blocker significantly suppressed this increase in Penh. We confirmed that intranasal OVA challenge did not induce bronchoconstriction by measuring airway resistance and bronchoalveolar lavage fluid, and through histological examination. These results clearly demonstrate that Penh could be a useful noninvasive indicator for studying nasal hypersensitivity.
DOI	10.1038/labinvest.3700452
PMID	16926838