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オクニシ カツヒデ
Okunishi Katsuhide
奥西 勝秀 所属 東邦大学 医学部 医学科 職種 教授 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa. |
| 掲載誌名 | 正式名：eLife ISSNコード：2050-084X |
| 掲載区分 | 国外 |
| 出版社 | ELIFE SCIENCES PUBLICATIONS LTD |
| 巻・号・頁 | 6,pp.e26174 |
| 著者・共著者 | Fushun Fan,Kohichi Matsunaga,Hao Wang,Ray Ishizaki,Eri Kobayashi,Hiroshi Kiyonari,Yoshiko Mukumoto,Katsuhide Okunishi,Tetsuro Izumi |
| 発行年月 | 2017/07/04 |
| 概要 | Exophilin-8 has been reported to play a role in anchoring secretory granules within the actin cortex, due to its direct binding activities to Rab27 on the granule membrane and to F-actin and its motor protein, myosin-Va. Here, we show that exophilin-8 accumulates granules in the cortical F-actin network not by direct interaction with myosin-Va, but by indirect interaction with a specific form of myosin-VIIa through its previously unknown binding partner, RIM-BP2. RIM-BP2 also associates with exocytic machinery, Cav1.3, RIM, and Munc13-1. Disruption of the exophilin-8-RIM-BP2-myosin-VIIa complex by ablation or knockdown of each component markedly decreases both the peripheral accumulation and exocytosis of granules. Furthermore, exophilin-8-null mouse pancreatic islets lose polarized granule localization at the β-cell periphery and exhibit impaired insulin secretion. This newly identified complex acts as a physical and functional scaffold and provides a mechanism supporting a releasable pool of granules within the F-actin network beneath the plasma membrane. |
| DOI | 10.7554/eLife.26174 |
| PMID | 28673385 |