コクラ　ケンジ   Kokura Kenji   古倉　健嗣    所属   東邦大学  理学部 生物分子科学科    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Cisplatin Induces Cell Death in Rat Adult Kidney Stem/ Progenitor Cell-Derived Kidney Organoids
掲載誌名	正式名：Yonago acta medica 略　 称：Yonago Acta Med ISSNコード：05135710/13468049
掲載区分	国内
出版社	Tottori University Medical Press
巻・号・頁	66(1),pp.153-158
総ページ数	6
著者・共著者	Yasushi Kuromi, Shinji Kitamura, Takeshi Endo, Shota Ueno, Kenji Kokura, Tetsuya Ohbayashi
発行年月	2023/02/16
概要	Background: The use of stem/ progenitor cell-derived organoids to evaluate the toxicity of chemical substances has widely increased. Organoids with nephron-like structures (NLS) can be derived from rat adult kidney stem/ progenitor cells (rKS cells) using three-dimensional culture. In this study, we examined the effects of cisplatin, an anticancer drug that induces nephrotoxicity in vivo, on rKS cell-derived NLS. Methods: Twelve organoids were simultaneously derived from three-dimensionally cultured rKS cells in Matrigel matrices. The surface area of each organoid was measured using microscopy-based imaging, and the morphological changes of NLS were monitored using an image analysis method. NLS were exposed to cisplatin, and their associated effects were assessed. Results: NLS elongated over time. The surface areas of the 12 organoids were almost constant. Cisplatin exposure induced cell death in NLS and inhibited the increase in the surface area of the organoids. Conclusion: Cisplatin exposure induces damage to NLS derived from rKS cells. Thus, the organoids may be valuable as an in vitro model to assess nephrotoxicity.