アマノ　ケンジ   Amano   天野　賢治    所属   東邦大学  医学部 医学科（大森病院）    職種   胚培養士
論文種別	原著
言語種別	英語
査読の有無	査読なし
表題	DSCAM deficiency causes loss of pre-inspiratory neuron synchroneity and perinatal death.
掲載誌名	正式名：The Journal of neuroscience : the official journal of the Society for Neuroscience
掲載区分	国外
巻・号・頁	29(9),pp.2984-96
著者・共著者	Kenji Amano,Morimitsu Fujii,Satoru Arata,Takuro Tojima,Masaharu Ogawa,Noriyuki Morita,Atsushi Shimohata,Teiichi Furuichi,Shigeyoshi Itohara,Hiroyuki Kamiguchi,Julie R Korenberg,Akiko Arata,Kazuhiro Yamakawa
発行年月	2009/03/04
概要	Down syndrome cell adhesion molecule (DSCAM) is a neural adhesion molecule that plays diverse roles in neural development. We disrupted the Dscam locus in mice and found that the null mutants (Dscam(-/-)) died within 24 h after birth. Whole-body plethysmography showed irregular respiration and lower ventilatory response to hypercapnia in the null mutants. Furthermore, a medulla-spinal cord preparation of Dscam(-/-) mice showed that the C4 ventral root activity, which drives diaphragm contraction for inspiration, had an irregular rhythm with frequent apneas. Optical imaging of the preparation using voltage-sensitive dye revealed that the pre-inspiratory neurons located in the rostral ventrolateral medulla and belonging to the rhythm generator for respiration, lost their synchroneity in Dscam(-/-) mice. Dscam(+/-) mice, which survived to adulthood without any overt abnormalities, also showed irregular respiration but milder than Dscam(-/-) mice. These results suggest that DSCAM plays a critical role in central respiratory regulation in a dosage-dependent manner.
DOI	10.1523/JNEUROSCI.3624-08.2009
PMID	19261893