アマノ　ケンジ   Amano Kenji   天野　賢治    所属   東邦大学  医学部 医学科（大森病院）    職種   胚培養士
論文種別	原著
言語種別	英語
査読の有無	査読なし
表題	Loss of pre-inspiratory neuron synchroneity in mice with DSCAM deficiency.
掲載誌名	正式名：Advances in experimental medicine and biology
掲載区分	国外
巻・号・頁	669,pp.15-9
著者・共著者	Kenji Amano,Morimitsu Fujii,Satoru Arata,Masaharu Ogawa,Kazuhiro Yamakawa,Akiko Arata
発行年月	2010
概要	Down syndrome cell adhesion molecule (DSCAM) is a neural adhesion molecule that plays diverse roles in neural development. We disrupted the Dscam locus in mice and found that the null mutants (Dscam (-/-)) died within 24 hours after birth. Whole body plethysmography showed irregular respiration and lower ventilatory response to hypercapnia in the null mutants. Further, a medulla-spinal cord preparation of Dscam (-/-) mice showed that the C4 ventral root activity, which drives diaphragm contraction for inspiration, had an irregular rhythm with frequent apneas. Optical imaging of the preparation using voltage-sensitive dye revealed that the pre-inspiratory (Pre-I) neurons located in the rostral ventrolateral medulla (RVLM) and belonging to the rhythm generator for respiration, lost their synchroneity in Dscam (-/-) mice. Dscam (+/-) mice, which survived to adulthood without any overt abnormalities, also showed irregular respiration but milder than Dscam (-/-) mice. These results suggest that DSCAM plays a critical role in central respiratory regulation in a dosage-dependent manner. These results have been published (Amano et al. 2009).
DOI	10.1007/978-1-4419-5692-7_3
PMID	20217312