オバラ　チズカ   Obara Chizuka   小原　千寿香    所属   東邦大学  理学部 生物学科    職種   博士研究員
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Establishment of an immunoscreening system using recombinant VP1 protein for the isolation of a monoclonal antibody that blocks JC virus infection.
掲載誌名	正式名：Biochemical and biophysical research communications ISSNコード：0006-291X
掲載区分	国外
出版社	ACADEMIC PRESS INC ELSEVIER SCIENCE
巻・号・頁	327(1),pp.242-51
著者・共著者	Chizuka Henmi,Hirofumi Sawa,Hiroshi Iwata,Yasuko Orba,Shinya Tanaka,Kazuo Nagashima
発行年月	2005/02/04
概要	Polyomavirus JC (JCV) infection causes the fatal human demyelinating disease, progressive multifocal leukoencephalopathy. Although the initial interaction of JCV with host cells occurs through direct binding of the major viral capsid protein (VP1) with cell-surface molecules possessing sialic acid, these molecules have not yet been identified. In order to isolate monoclonal antibodies which inhibit attachment of JCV, we established an immunoscreening system using virus-like particles consisting of the VP1. Using this system, among monoclonal antibodies against the cell membrane fraction from JCV-permissive human neuroblastoma IMR-32 cells, we isolated a monoclonal antibody designated as 24132 that specifically inhibited attachment and infection of JCV to IMR-32 cells. The antibody 24132 recognized a single molecule of around 60 kDa in molecular weight in the IMR-32 membrane fraction. Immunohistochemical staining with 24132 demonstrated immunoreactivity in the cell membrane of JCV-permissive cell lines and glial cells of the human brain. These results suggested that the molecule recognized by 24132 plays a role in JCV infection, and that it might participate as a receptor or a co-receptor in JCV attachment and entry into the cells. (C) 2004 Elsevier Inc. All rights reserved.
DOI	10.1016/j.bbrc.2004.11.158
PMID	15629455