オバラ　チズカ   Obara Chizuka   小原　千寿香    所属   東邦大学  理学部 生物学科    職種   博士研究員
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Genetic aberrations in iPSCs are introduced by a transient G1/S cell cycle checkpoint deficiency.
掲載誌名	正式名：Nature communications
掲載区分	国外
巻・号・頁	11(1),pp.197-197
著者・共著者	Ryoko Araki,Yuko Hoki,Tomo Suga,Chizuka Obara,Misato Sunayama,Kaori Imadome,Mayumi Fujita,Satoshi Kamimura,Miki Nakamura,Sayaka Wakayama,Andras Nagy,Teruhiko Wakayama,Masumi Abe
発行年月	2020/01/10
概要	A number of point mutations have been identified in reprogrammed pluripotent stem cells such as iPSCs and ntESCs. The molecular basis for these mutations has remained elusive however, which is a considerable impediment to their potential medical application. Here we report a specific stage at which iPSC generation is not reduced in response to ionizing radiation, i.e. radio-resistance. Quite intriguingly, a G1/S cell cycle checkpoint deficiency occurs in a transient fashion at the initial stage of the genome reprogramming process. These cancer-like phenomena, i.e. a cell cycle checkpoint deficiency resulting in the accumulation of point mutations, suggest a common developmental pathway between iPSC generation and tumorigenesis. This notion is supported by the identification of specific cancer mutational signatures in these cells. We describe efficient generation of human integration-free iPSCs using erythroblast cells, which have only a small number of point mutations and INDELs, none of which are in coding regions.
DOI	10.1038/s41467-019-13830-x
PMID	31924765
PermalinkURL	https://www.nature.com/articles/s41467-019-13830-x#citeas