ヤマグチ シンペイ   Yamaguchi Shinpei
  山口 新平
   所属   東邦大学  理学部 生物学科
   職種   准教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 A region-dependent allele-biased expression of Dopa decarboxylase in mouse brain.
掲載誌名 正式名:Frontiers in cell and developmental biology
掲載区分国外
巻・号・頁 10,pp.1078927-1078927
著者・共著者 Kit-Yeng Sheng,Toru Nakano,Shinpei Yamaguchi
担当区分 最終著者,責任著者
発行年月 2022/12/07
概要 Genomic imprinting is an epigenetic event in which genes are expressed only from either the paternal or maternal allele. Dopa decarboxylase (Ddc), is an imprinted gene that encodes an enzyme which catalyzes the conversion of L-dopa to dopamine. Although Ddc has been reported to be paternally expressed in embryonic and neonatal hearts, its expression pattern in the brain has been controversial. To visualize Ddc-expressing neurons, we established a knock-in mouse carrying a humanized Kusabira orange 1 (hKO1) reporter cassette at the Ddc locus (Ddc-hKO1). The expression of Ddc-hKO1 was detected in all known Ddc-positive cells in the brains of embryonic, neonatal, adult, and aged mice. We further developed an efficient purification method for Ddc-hKO1-positive neurons using a cell sorter. RNA sequencing analysis confirmed the enrichment of dopaminergic, serotonergic and cholinergic neurons in Ddc-hKO1-positive cell population recovered using this method. A detailed analysis of Ddc-hKO1 paternally and maternally derived heterozygous mice combined with immunostaining revealed that Ddc was preferentially expressed from the maternal allele in ventral tegmented area (VTA), substantia nigra pars compacta (SNc), and retrorubral field (RRF); while it was expressed from both alleles in dorsal raphe nucleus (DR). These results indicate that Ddc exhibit an allele-specific expression pattern in different brain regions, presumably reflecting the diverse regulatory mechanisms of imprinting.
DOI 10.3389/fcell.2022.1078927
PMID 36568970