イシダ　マサヒロ   Ishida Masahiro   石田　政弘    所属   東邦大学  医学部 医学科（大橋病院）    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Optimization of non-isotopic in situ hybridization: Detection of the Y chromosome in paraformaldehyde-fixed, wax-embedded cat retina
掲載誌名	正式名：EXPERIMENTAL EYE RESEARCH ISSNコード：00144835
出版社	ACADEMIC PRESS LTD
巻・号・頁	66(2),pp.223-230
著者・共著者	RH Yao,IK Sugino,KM Greulich,M Ishida,HUG Weier,MA Zarbin
発行年月	1998/02
概要	A technique was developed to detect the Y chromosome in paraformaldehyde-fixed diethylglycoldiesterate-embedded cat retina. The Y chromosome specific DNA probe was labeled with digoxigenin through polymerase chain reaction incorporation. After treatment of paraformaldehyde-fixed, diethylglycoldiesterate-embedded tissue sections with deoxyribonucleic acid decondensation and proteolytic digestion, non-fluorescent, non-isotopic in situ hybridization was performed on the retina sections. Most extensive treatment was required for the outer nuclear layer while the inner nuclear layer required more extensive treatment than the retinal pigment epithelial cells. Under optimal pretreatment conditions, the male cat retina displayed black spots which specifically localized at the periphery of the nuclei, while the female cat retina showed negative staining for the Y chromosome specific probe. The technique allows observation of the Y chromosome signal with preservation of retinal morphology and thus may be a valuable tool to discriminate donor cells in retinal pigment epithelial cell and photoreceptor cell transplants. (C) 1998 Academic Press Limited.
DOI	10.1006/exer.1997.0427
PMID	9533848