イシダ　マサヒロ   Ishida Masahiro   石田　政弘    所属   東邦大学  医学部 医学科（大橋病院）    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Culture of human retinal pigment epithelial cells from peripheral scleral flap biopsies
掲載誌名	正式名：CURRENT EYE RESEARCH ISSNコード：02713683
出版社	AEOLUS PRESS
巻・号・頁	17(4),pp.392-402
著者・共著者	M Ishida,GM Lui,A Yamani,IK Sugino,MA Zarbin
発行年月	1998/04
概要	Purpose. We studied various methods for harvesting retinal pigment epithelium (RPE) biopsies from cadaverhuman eyes of donors over age 60 years. Our goal was to harvest cells for possible autologous RPE cell transplantation in patients with age-related macular degeneration and to test the viability of the RPE after isolation by evaluating explant growth in culture,Methods. Choroid-RPE biopsies were excised from enucleated human eyes. The RPE was separated from the choroid by treatment with type TV collagenase. RPE patches were cultured. After 100-500 cells had grown out from the explant, the primary cultures were passaged.Results. There was no clear effect of donor age on the ability to establish primary RPE cultures with good morphology from biopsies 2 x 2-10 x 10 mm(2) in size. Biopsies 6 x 6 mm(2) or larger produced satisfactory primary cultures more than 70% of the time. The number of viable RPE cells (defined as the number of cells adherent to the culture dish 24 h after plating obtained after enzymatic separation of the RPE and choroid was an important determinant of our ability to establish primary cultures and passage the cells. Primary cultures with good cellular morpho
PMID	9561831