イシダ　マサヒロ   Ishida Masahiro   石田　政弘    所属   東邦大学  医学部 医学科（大橋病院）    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Phototoxicity of Indocyanine Green under Continuous Fluorescent Lamp Illumination and Its Prevention by Blocking Red Light on Cultured Muller Cells
掲載誌名	正式名：INVESTIGATIVE OPHTHALMOLOGY&VISUAL SCIENCE ISSNコード：01460404
出版社	ASSOC RESEARCH VISION OPHTHALMOLOGY INC
巻・号・頁	51(8),pp.4337-4345
著者・共著者	Tomohito Sato,Masataka Ito,Masahiro Ishida,Yoko Karasawa
発行年月	2010/08
概要	PURPOSE. To investigate the phototoxicity of persistent indocyanine green (ICG) under continuous visible light illumination and to determine whether blocking peak absorbance wavelengths of ICG is cytoprotective.METHODS. Cultured quail Muller cells were exposed to 0 to 5 mg/mL ICG for 30 seconds or 10 minutes and then were cultured in a colorless medium for 24 hours with or without continuous fluorescent lamp illumination. Cells exposed to 5 mg/mL ICG for 10 minutes were cultured under illumination filtered through a dichroic mirror that blocks red to near-infrared, green, or blue wavelengths. After microscopic observation, cell viability and cell death were evaluated.RESULTS. ICG exposure followed by illuminated culture induced severe morphologic changes in cells, significant reductions in cell viability, and increases in cell death from apoptosis compared with exposure to ICG or illumination alone or with no exposure. Although ICG exposure at higher concentrations caused cell damage in a dose-and time-dependent manner, an increase in cell viability was noted for cells exposed to lower ICG concentrations. Blocking red to near-infrared wavelengths prevented the decrease in cell vi
DOI	10.1167/iovs.09-4707
PMID	20335621