イノウエ　エイジ   Inoue Eiji   井上　英治    所属   東邦大学  理学部 生物学科    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Sex Identification and Efficient Microsatellite Genotyping Using Fecal DNA in Proboscis Monkeys (Nasalis larvatus)
掲載誌名	正式名：Mammal Study
巻・号・頁	41(3),pp.141-148
著者・共著者	Eiji Inoue＊†, Mitsuaki Ogata, Satoru Seino, Ikki Matsuda
担当区分	筆頭著者
発行年月	2016/09
概要	Genetic sex identification and efficient microsatellite genotyping using non-invasive samples are important for assessing genetic diversity and structure of wild mammals. We extracted DNA from fecal samples of endangered proboscis monkeys (Nasalis larvatus) at the Yokohama Zoological Gardens, and successfully identified their sex using a DEAD-box gene marker. Using the information of 31 published microsatellite loci developed for proboscis monkeys, we redesigned the primers for 18 loci and selected microsatellite loci with high heterozygosities and then developed a multiplex PCR system, in which 19 polymorphic microsatellite loci and a sex identification marker were amplified in three multiplex sets. In our developed method, genotyping errors rarely happened using DNA extracted from feces, and we could identify individuals and estimate parent—offspring relationship with high confidence. Our developed method is applicable for genetic analysis of wild proboscis monkeys and will provide us the information of not only their ecological and social features but also conservation management.
DOI	http://dx.doi.org/10.3106/041.041.0304