ミカミ　ヨシノリ   Mikami Yoshinori   三上　義礼    所属   東邦大学  医学部 医学科    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Chlorogenic acid, a polyphenol in coffee, protects neurons against glutamate neurotoxicity
掲載誌名	正式名：Life sciences 略　 称：Life Sci ISSNコード：00243205
巻・号・頁	139(1),pp.69-74
著者・共著者	Mikami Y†, Yamazawa T＊
担当区分	筆頭著者
発行年月	2015/10
概要	AIMS: The present study has been designed to explore the molecular mechanism of chlorogenic acid (CGA) in the protective effect against glutamate-induced neuronal cell death. MAIN METHODS: Cortical neurons in primary culture were exposed to 300 muM l-glutamic acid or vehicle, with or without 10 muM CGA or 10 muM MK-801. After 16 h, primary cultures were stained with propidium iodide (PI)/Hoechst or calcein. Double-staining with PI and Hoechst was performed to confirm whether cell death induced by glutamate was apoptotic. In addition, intracellular concentrations of Ca2+ were observed using Ca2+ indicator fura-2. KEY FINDINGS: We investigated the protective effects of CGA on glutamate-induced neuronal cell death using primary cultures of mouse cerebral cortex because the release of glutamate during brain ischemia triggers death of neurons. Glutamate-induced neuronal cell death was inhibited by treatment with CGA. In addition, CGA prevented the increase in intracellular concentrations of Ca(2+) caused by the addition of glutamate to cultured neurons. On the other hand, there was little effect of CGA on cell death induced by nitric oxide, which is downstream of the ischemic neuronal cell death. Our results suggested that the polyphenol CGA in coffee protects neurons from glutamate neurotoxicity by regulating Ca2+entry into neurons. SIGNIFICANCE: CGA in coffee may have clinical benefits for neurodegenerative diseases such as ischemic stroke.
DOI	10.1016/j.lfs.2015.08.005