タカハシ　ケンイチロウ   Takahashi Ken-Ichiro   髙橋　謙一郎    所属   東邦大学  医学部 医学科（大森病院）    職種   助教
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	The high-temperature requirement factor A3 (HtrA3) is associated with acquisition of the invasive phenotype in oral squamous cell carcinoma cells.
掲載誌名	正式名：Oral oncology 略　 称：Oral Oncol ISSNコード：13688375
巻・号・頁	51(1),pp.84-89
著者・共著者	Moriya Y, Uzawa N, Morita T, Mogushi K, Miyaguchi K, Takahashi K, Michikawa C, Sumino J, Tanaka H, Harada K.
発行年月	2015/01
概要	OBJECTIVES: Previous studies have identified several genes involved in the carcinogenesis of oral cancer; however, the detailed mechanisms underlying this process have not been elucidated. Previously, we established a database of the transcriptional progression profile of oral carcinogenesis and identified 15 candidate genes with continuously increasing or decreasing expression (Sumino et al., 2013). MATERIALS AND METHODS: In the present study, using this database, we attempted to identify genes that may specifically contribute to progression from oral dysplastic lesions to invasive tumours. RESULTS: We identified 4 candidate genes. Using a literature survey, we narrowed down the candidates and focused on the high-temperature requirement factor A3 (HtrA3). Quantitative real-time reverse transcription polymerase chain reaction and immunohistochemical analysis confirmed that HtrA3 expression significantly increased during this process. In addition, high HtrA3 expression was significantly associated with decreased disease-free survival (P=0.045) and overall survival (P=0.003). Multivariate Cox proportional hazards analysis found that high HtrA3 expression significantly correlated with overall survival (P=0.018). CONCLUSION: The findings of this study demonstrated that the HtrA3 is likely to be associated with the acquisition of the invasive phenotype in oral squamous cell carcinoma cells and may be a potential prognostic marker for oral cancer.