アリタ ミチツネ
Arita Michitsune
有田 通恒 所属 東邦大学 医学部 医学科 職種 助教 |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | A single nucleotide substitution (-107C G) in hMLH1 promoter found in colorectal cancer population reduces transcriptional activity |
掲載誌名 | 正式名:Biochemical genetics 略 称:Biochem Genet ISSNコード:00062928 |
掲載区分 | 国外 |
巻・号・頁 | 45(9-10),pp.671-681 |
著者・共著者 | Zhong X†, Arita M†, Yamada K†, Sugiyama H, Tan K†, Kanazawa S, Koike J, Teramoto T, Hemmi, H*† |
担当区分 | 2nd著者 |
発行年月 | 2007/10 |
概要 | Inactivation of the DNA mismatch repair gene hMLH1 predisposes one to colorectal cancer. We have identified a C to G nucleotide substitution at position -107 relative to the hMLH1 gene translation initiation site in three of 163 colorectal cancer patients with an allele frequency of 0.0092 (3/326). One of the three -107G alleles occurred in one patient out of five with reduced hMLH1 expression in the tumor tissue. The -107G was not found in 63 healthy individuals. This substitution reduced transcriptional activity by 51% compared with -107C (P<0.01) and impeded the promoter-binding capacity of nuclear proteins. Although the small number of identified -107G alleles is insufficient to evaluate the contribution to the carcinogenesis and clinicopathological properties of the tumors, the effects of -107G on hMLH1 gene transcription and nuclear protein binding to the promoter sequence implicate the site, including -107C, as a crucial element interacting with the activator that maintains hMLH1 gene expression. |
文献番号 | PMID: 17690979 |
researchmap用URL | https://researchmap.jp/0713/ |