スギモト　マサズミ   Sugimoto Masazumi   杉本　雅純    所属   東邦大学  理学部 生物分子科学科    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Molecular cloning and characterization of a new RGS protein of Medaka
掲載誌名	正式名：Gene 略　 称：Gene ISSNコード：03781119
巻・号・頁	345(2),pp.165-171
著者・共著者	Itoh M, Nagatomo K, Kubo Y, Sugimoto M, Saitoh O
発行年月	2005/01
概要	We identified eight genes of putative RGS proteins in skin of Medaka fish using PCR amplification with degenerate primers for the RGS domain of known RGS proteins. Then, we cloned a full-length cDNA for a new RGS protein. This RGS protein was similar to human RGS3 within the RGS domain, but other parts were unique among known RGS proteins. RT-PCR analysis demonstrated that this Medaka RGS3-like protein (MeRGS3L) is mainly expressed in skin and heart. When coexpressed in Xenopus oocytes, MeRGS3L accelerated the turning-on and -off of Gi/o-mediated modulation of GIRK channels without apparent desensitization in the presence of ligand. MeRGS3L also decreased the response of Gq signaling upon activation of m1 muscarinic receptor. This new RGS protein may play important roles in regulation of melanophore responses in Medaka skin.