ニシオ　ジュンコ   Nishio Junko   西尾　純子    所属   東邦大学  医学部 医学科    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Revisiting the role of IRF3 in inflammation and immunity by conditional and specifically targeted gene ablation in mice.
掲載誌名	正式名：Proceedings of the National Academy of Sciences of the United States of America
掲載区分	国外
巻・号・頁	115(20),pp.5253-5258
著者・共著者	Hideyuki Yanai,Shiho Chiba,Sho Hangai,Kohei Kometani,Asuka Inoue,Yoshitaka Kimura,Takaya Abe,Hiroshi Kiyonari,Junko Nishio,Naoko Taguchi-Atarashi,Yu Mizushima,Hideo Negishi,Rudolf Grosschedl,Tadatsugu Taniguchi
発行年月	2018/05/15
概要	IFN regulatory factor 3 (IRF3) is a transcription regulator of cellular responses in many cell types that is known to be essential for innate immunity. To confirm IRF3's broad role in immunity and to more fully discern its role in various cellular subsets, we engineered Irf3-floxed mice to allow for the cell type-specific ablation of Irf3 Analysis of these mice confirmed the general requirement of IRF3 for the evocation of type I IFN responses in vitro and in vivo. Furthermore, immune cell ontogeny and frequencies of immune cell types were unaffected when Irf3 was selectively inactivated in either T cells or B cells in the mice. Interestingly, in a model of lipopolysaccharide-induced septic shock, selective Irf3 deficiency in myeloid cells led to reduced levels of type I IFN in the sera and increased survival of these mice, indicating the myeloid-specific, pathogenic role of the Toll-like receptor 4-IRF3 type I IFN axis in this model of sepsis. Thus, Irf3-floxed mice can serve as useful tool for further exploring the cell type-specific functions of this transcription factor.
DOI	10.1073/pnas.1803936115
PMID	29712834