ニシオ　ジュンコ   Nishio Junko   西尾　純子    所属   東邦大学  医学部 医学科    職種   准教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Identification and characterization of a novel Enterococcus bacteriophage with potential to ameliorate murine colitis
掲載誌名	正式名：Scientific Reports ISSNコード：/2045-2322
出版社	Springer Science and Business Media LLC
巻・号・頁	11(1)
著者・共著者	Junko Nishio,Hideo Negishi,Mika Yasui-Kato,Shoji Miki,Kazuhiko Miyanaga,Kotaro Aoki,Takuma Mizusawa,Masami Ueno,Akira Ainai,Masafumi Muratani,Sho Hangai,Hideyuki Yanai,Hideki Hasegawa,Yoshikazu Ishii,Yasunori Tanji,Tadatsugu Taniguchi
担当区分	筆頭著者
発行年月	2021/10/12
概要	Abstract Increase of the enteric bacteriophages (phage), components of the enteric virome, has been associated with the development of inflammatory bowel diseases. However, little is known about how a given phage contributes to the regulation of intestinal inflammation. In this study, we isolated a new phage associated with Enterococcus gallinarum, named phiEG37k, the level of which was increased in C57BL/6 mice with colitis development. We found that, irrespective of the state of inflammation, over 95% of the E. gallinarum population in the mice contained phiEG37k prophage within their genome and the phiEG37k titers were proportional to that of E. gallinarum in the gut. To explore whether phiEG37k impacts intestinal homeostasis and/or inflammation, we generated mice colonized either with E. gallinarum with or without the prophage phiEG37k. We found that the mice colonized with the bacteria with phiEG37k produced more Mucin 2 (MUC2) that serves to protect the intestinal epithelium, as compared to those colonized with the phage-free bacteria. Consistently, the former mice were less sensitive to experimental colitis than the latter mice. These results suggest that the newly isolated phage has the potential to protect the host by strengthening mucosal integrity. Our study may have clinical implication in further understanding of how bacteriophages contribute to the gut homeostasis and pathogenesis.
DOI	10.1038/s41598-021-99602-4
PMID	34642357
PermalinkURL	https://www.nature.com/articles/s41598-021-99602-4.pdf
researchmap用URL	https://www.nature.com/articles/s41598-021-99602-4