イシダ　マサヒロ   Ishida Masahiro   石田　政弘    所属   東邦大学  医学部 医学科（大橋病院）    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Preparation and transplantation of photoreceptor sheets
掲載誌名	正式名：CURRENT EYE RESEARCH ISSNコード：02713683
出版社	AEOLUS PRESS
巻・号・頁	17(6),pp.573-585
著者・共著者	JC Huang,M Ishida,P Hersh,IK Sugino,MA Zarbin
発行年月	1998/06
概要	Purpose. Photoreceptor(PR) transplantation may be a treatment for blindness secondary to PR degeneration. We studied different technical aspects of PR-sheet preparation.Methods. Geographic variation in the thickness of the cat PR layer (from the outer segments to the outer plexiform layer) and inner retina (width of the remainder of the retina) was studied. PR sheets (cat and human) were prepared through gelatin embedding and subsequent vibratoming or excimer laser ablation. Cat PR sheets were evaluated after transplantation.Results. The thickness of the cat PR layer and inner retina varied in different regions. The superior central retina, including the area centralis, was thickest (PR layer: 115-123 mu m, entire retina: 225-230 mu m, in fixed tissue). The peripheral retina was similar to 40% thinner than the center. Fresh retina was similar to 7.9% thicker than the fixed retina. Both vibratomy and excimer laser ablation removed the inner retina, leaving a PR-layer sheet with good morphology. To produce good quality PR sheets with vibratomy, use of different gelatin concentrations (2% to 35%) at various stages of sheet preparation was crucial. To produce PR sheets of uniform th
PMID	9663847