東邦大学 教育・研究業績データベース   
     


  イトウ マサノリ   Ito Masanori
  伊藤 雅方
   所属   東邦大学  医学部 医学科
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Design of hammerhead ribozymes that cleave murine Sry mRNA in vitro and in vivo.
掲載誌名 正式名:Journal of Reproduction and Development
略  称:J Reprod Dev
ISSNコード:0916-8818/1348-4400
インパクトファクター 1.149
巻・号・頁 52(1),73-80頁
著者・共著者 Nakamura K, Murata C, Ito M, Iwamori T, Nishimura S, Hisamatsu S, Sonoki S, Nakayama A, Suyama E, Kawasaki H, Taira K, Nishino K, Tachi C
発行年月 2006/02
概要 As the first step in investigating the possiblity of applying ribozyme technology to artificial control of the sex ratios at birth in farm animals, where the demand for females exceeds that for males, we designed a hammerhead ribozyme (HHRz) and 2 tRNA(val)-hammerhead ribozyme complexes (tRNARz3 and tRNARz4), and examined their effects upon murine Sry mRNA in vitro and in cells. We demonstrated that HHRz and tRNARz3 could effectively cleave the target Sry mRNA in vitro. For the purpose of experiments in vivo, HHRz was cloned into the highly efficient pUC-CAGGS mammalian expression vector (pCAG/HHRz), and the tRNA ribozyme complexes were cloned into the pol III promoter-driven pPUR-KE vector (pPUR/tRNARz3 and pPUR/tRNARz4); the ribozyme vectors were co-transfected with the target vector (pCAG/Sry). A suppressive action (up to approx. 60%) was confirmed for pCAG/HHRz and pPUR/tRNARz3 upon the transiently expressed exogenously introduced Sry in M15 cultured cells.
DOI 10.1262/jrd.17060
文献番号 16293944