東邦大学 教育・研究業績データベース   
     


  イトウ マサノリ   Ito Masanori
  伊藤 雅方
   所属   東邦大学  医学部 医学科
   職種   講師
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Detection of elements responsible for stage- and tissue-specific expression of mouse Sry using an in vitro Cre/loxP system.
掲載誌名 正式名:Biochemical Biophysical Research Communications
略  称:Biochem Biophys Res Commun
インパクトファクター 3
巻・号・頁 337(1),264-270頁
著者・共著者 Ito M, Yokouchi K, Naito K, Endo H, Hakamata Y, Miyazaki J, Tojo H
発行年月 2005/11
概要 We have successfully specified essential sequences of the 5' upstream region for the stage- and tissue-specific expression of mouse Sry by using an in vitro Cre/loxP system. Sry/Cre plasmids carrying Sry 5' sequences of various sizes were transfected into the primary cultured cells from different tissues of CAG/loxP/CAT/loxP/LacZ transgenic fetuses on 11.5-day post coitus (dpc) or 13.5-dpc. Stage- and tissue-specific regulation of Sry expression was disrupted by the deletion of positions 7549-7660 (from -0.4 to -0.5 kb region). In vitro transcription assay also suggested that the region contains element(s) responsible for stage- and tissue-specific expression of mouse Sry. SRY promoter of Shiba goat (Capra hircus var Shiba), a native Japanese miniature goat, showed the tissue-specific activity in the cells from urogenital ridges of the male mouse, but not in the cells from female mice, indicating a possibly different mechanism among species in the regulation of Sry expression.
DOI 10.1016/j.bbrc.2005.09.047
文献番号 16182245