ミズノ　フヅキ   Mizuno Fuzuki   水野　文月    所属   東邦大学  医学部 医学科    職種   講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Emulsion PCR-coupled target enrichment: An effective fishing method for high-throughtput sequencing of poorly preserved ancient DNA.
掲載誌名	正式名：Gene ISSNコード：03781119
掲載区分	国外
巻・号・頁	528(2),pp.347-351
著者・共著者	Kihana M#, Mizuno F#, Sawafuji R, Wang L, Ueda S
担当区分	筆頭著者
発行年月	2013/07
概要	Due to the difficulties in deep sequencing, high-throughput sequencing of ancient DNA has been limited to exceptionally well-preserved ancient materials. The primary factor is microbial attack popularly observed in the buried materials, and it causes drastic increase in relative ratio of microbial DNA in the extracted DNA. We present a unified strategy in which emulsion PCR is coupled with target enrichment followed by next-generation sequencing. The method made it possible to obtain efficiently non-duplicated reads mapped to target sequences of interest, and this can achieve deep and reliable sequencing of ancient DNA from typical materials, even though poorly preserved.
DOI	doi: 10.1016/j.gene.2013.07.040.
PMID	23900195